Figure 1.

MutFUS mislocalizes to the cytoplasm and increases GFAP expression in primary astrocytes: (a) Immunofluorescence staining of astrocytes infected with GFP‐FUS, 5 days after infection, for GLAST and DAPI. Scale bar 30 μm. (b) Quantification of percentage of astrocytes displaying cytoplasmic GFP‐FUS. Statistical significance was evaluated using Student's t test, n = 3, *p < 0.05 (c) Western blots of astrocytic total lysate confirming equal levels of expression: top GFP, middle actin. (d) Immunofluorescence staining of astrocytes infected with GFP‐FUS, 5 days after infection, for GFAP. (e) Western blot of total astrocytic lysate: top GFAP, bottom GAPDH; lower panel, GFP‐FUS. (f) Quantification of GFAP levels displayed in Panel (e). Densitometry data are presented as mean ± SEM. GFAP levels were normalized to the GAPDH loading control and presented relative to nontransduced control. Statistical significance was evaluated using one‐way ANOVA, n = 3, *p < 0.01