Figure 2.

MutFUS‐expressing astrocytes are toxic to motor neurons: (a) Motor neurons plated on a bed of mutFUS‐infected astrocytes displayed shorter neurites. Immunostained for p75NTR and DAPI. (b) Tracings of co‐cultured motor neurons on a bed of virally infected FUS astrocytes. Scale bar 10 μm. (c) Representative cumulative frequency graph show motor neurons co‐cultured with MutFUS astrocytes exhibit a reduction in average neurite length. WTSOD1 and MutSOD1 used for comparison due to their known effects on motor neurons. Average neurite length of each condition shown as vertical dotted line. >100 neurons traced per condition. (d) Neurite length data are presented as mean ± SEM. Data were assessed after a logarithmic transformation to normalize the distribution and statistical significance was evaluated using Student's t test, >100 neurons traced per condition, n = 4, *p < 0.05. The data shown are the raw untransformed data in the original units