Figure 5.

TNFα neutralization in MutFUS ACM rescues neurite loss and motor neuron death: (a) Tracings made of individually tracked live motor neurons, cultured in isolation, and treated with ACM. TNFα neutralization antibody (nAb) diluted directly into ACM. (b) Representative cumulative frequency of neurite lengths of motor neurons treated with ACM and TNFα nAb. >100 neurons traced per condition. (c) Neurite length data are presented as mean ± SEM. Statistical significance was evaluated using one‐way ANOVA, >100 neurons traced per condition, n = 4, *p < 0.05. (d) Quantification of the percentage of motor neurons remaining after 96 hr of ACM addition. Survival data are presented as mean ± SEM and statistical significance was evaluated by one‐way ANOVA, >50 neurons per condition, n = 3, *p < 0.01