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Identification of circulating tumor cells (CTCs). Cells that were CD45‐positive (a) and had ≤2 chromosome 8 centromeres (CEP8) (b) were deemed not to be CTCs. Hyperdiploid cells in peripheral blood samples with no CD45‐positive staining were identified CTCs; shown are a triploid CTC (c), tetraploid CTC (d), and multiploid CTC (e). DAPI, 4′,6‐diamidino‐2‐phenylindole
