Figure 2.

Suitability of the modified 384‐well‐device for substance screenings. Scheme and photo documentation of PaTu‐8988t wildtype cells cultivated 48 h with LO‐ (A) and MHD‐method (B). Dose‐response‐relations and calculated IC₅₀‐values of PaTu‐8988t wildtype cells treated with gemcitabine. Several independent approaches (n) were performed manually with LO‐method ($\square$, n = 7) or automatically with LO‐ (◇, n = 6) or MHD‐method (△, n = 5). Manually performed analyses with 2D cultures (×, n = 2) serve as control (C). Automated microscopic determination of HT29‐cells cultivated 2D or 3D with LO‐ and MHD‐method and calculated IC₅₀‐values from these cultures treated with paclitaxel in two independent approaches (D). Dose‐response‐relation and calculated IC₅₀‐values of two doxycycline‐inducible mutants from PaTu‐8988t: clone I (E) and clone II (F) treated with gemcitabine in two independent approaches (LO‐method automatically ▴ and manually •). Data are depicted as mean +/‐ SD with 12 samples contributing to each concentration of every individual approach n. Bar – 500 μm.