Table 1.
Antibodies used for immunohistochemical evaluation of 42 cases of nasopharyngeal angiofibroma from Guatemala
| Antibody | Source/clone | Dilution | Antigen retrieval |
|---|---|---|---|
| Vimentin | Dako/Vim3B4 | 1:400 | Citrate buffer (pH 6.0) |
| CD34 | Dako/QBEnd10 | 1:50 | Citrate buffer (pH 6.0) |
| CD31 | Dako/1C70A | 1:200 | EDTA/TRIS (pH 9.0) |
| FVIII (Von Willebrand Factor) | Dako/F8/86 | 1:100 | Citrate buffer (pH 6.0) |
| CD105 | Dako/SNGH | 1:30 | Proteinase K |
| Podoplanin | Dako/D2-40 | 1:100 | Citrate buffer (pH 6.0) |
| Desmin | Dako/D33 | 1:800 | Citrate buffer (pH 6.0) |
| Smooth muscle actin | Dako/1A4 | 1:400 | Citrate buffer (pH 6.0) |
| Muscle specific actin | Dako/HHF-35 | 1:800 | Citrate buffer (pH 6.0) |
| H-caldesmon | Dako/N-CD | 1:400 | Citrate buffer (pH 6.0) |
| Calponin | Dako/CALP | 1:600 | Citrate buffer (pH 6.0) |
| Mast cell tryptase | Dako/AA1 | 1:10,000 | Citrate buffer (pH 6.0) |
| CD1a | Dako/010 | 1:600 | Citrate buffer (pH 6.0) |
| S100 | Dako/polyclonal | 1:10,000 | Citrate buffer (pH 6.0) |
| Factor XIIIa | Novocastra/E980.1 | 1:100 | EDTA/TRIS (pH 9.0) |
| CD68 | Dako/PGM1 | 1:400 | Citrate buffer (pH 6.0) |
| CD163 | Novocastra/10D6 | 1:4000 | Citrate buffer (pH 6.0) |
| Ki-67 | Dako/Mib-1 | 1:100 | Citrate buffer (pH 6.0) |