Fig. 1.

Direct RT-RPA detection method workflow showing sample processing and assay setup steps. 1 - punch leaf with lid of 1.5 mL tubes; 2 - immerse leaf disk in alkaline-PEG buffer and incubate at room temperature for <5 min; 3 - resuspend lyophilised pellet; 4 - read fluorescence; 5 - analyse results: an increase in fluorescence above threshold indicates a positive reaction.