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. 2018 Apr 1;546:17–22. doi: 10.1016/j.ab.2018.01.019

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© 2018 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 2 — RT-RPA, with purified RNAs as template and Direct RT-RPA, with crude plant extracts as template for detection of YMV (a.) and YMMV (b.). Blue lines are obtained from purified RNAs and red lines obtained from crude plant extracts. Circle corresponds to Idu-Ekpeye (TDr) and TDa 98/159 samples; Triangle corresponds to TDr 02/00515 and TDa 00/00005 samples; Cross corresponds to TDr 95/19177 and TDa 00/00194 samples. NTC refers to the non-template control. Fluorescence intensities were plotted against time in minutes. The solid bar corresponds to the threshold line (graph generated by CFX ManagerTM software). Tables on the right side of the amplification plots show the threshold times (min) of each reaction. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)