Figure 2.
Synapse-Specific STDP Induced Using Light Stimulation during Down States In Vivo
(A) Diagram of the protocols applied: top, control; middle, protocol 1 (pre-post); bottom, protocol 2 (post-pre).
(B) Examples of each of the three experiments in (A). Symbols show 1-min means of the EPSP slopes normalized to the 10-min baseline.
(C) Representative mean traces from the 10th (1, 3, and 5) and 50th min (2, 4, and 6) of recording.
(D) Evoked EPSPs were stable over 50 min (<0.1 Hz, control; n = 13 cells in N = 10 mice, mean ± SEM). A pre-post pairing protocol applied during Down states led to t-LTP (n = 8 neurons in N = 8 mice, mean ± SEM), while a post-pre pairing protocol led to t-LTD (n = 5 neurons in N = 5 mice, mean ± SEM).
(E) Summary of the results in (D). Number of recordings indicated in parentheses. Mean EPSP slope from last 5 min of recording was normalized to mean EPSP slope of the last 5 min of the baseline. The box and whisker plots represent maximum, upper quartile, mean (cross), median, lower quartile, and minimum values. One-way ANOVA and Dunnett’s post hoc test, ∗p < 0.05, ∗∗p < 0.01.