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. Author manuscript; available in PMC: 2018 Nov 21.
Published in final edited form as: Mol Biosyst. 2017 Nov 21;13(12):2704–2714. doi: 10.1039/c7mb00365j

Figure 5.

Figure 5

AgrA is required by S. aureus for EV production and activity against A. baumannii. a) Biofilm formation by S. aureus JE2 and an isogenic ΔagrA mutant strain. b) Protein content within concentrated samples from S. aureus JE2 and its isogenic ΔagrA mutant. S. aureus ATCC 25923 was included for comparison. The EVs vesicles in each culture were purified as described in the Materials and Methods, after which the protein content was determined using the Bradford Assay. Every attempt with the ΔagrA mutant found no protein after the purification was complete. c) TEM images of the two S. aureus strains, JE2 and its isogenic ΔagrA mutant, showing the mutant is not producing EVs. Numerous EVs are evident as they bud off of the wild-type JE2 cells. The purified EVs from this strain are shown in the middle image. In contrast, the JE2 ΔagrA cells do not produce EVs. d) A. baumannii biofilm formation in the presence of S. aureus JE2 supernatant (10% v:v) or that from from its isogenic ΔagrA mutant (a = p<0.001, b = p<0.05).