Skip to main content
. 2018 Mar 28;14(3):e1007270. doi: 10.1371/journal.pgen.1007270

Fig 3. HSF1 depletion induces filamentation independently of changes in HSP90 expression.

Fig 3

Strains were grown in the absence or presence of 20 μg/mL DOX in rich medium at 30°C. a) A strain was engineered where HSP90 is under the control of a constitutive promoter, ACT1p, in the tetO-HSF1/hsf1Δ background. In the ACT1p-HSP90 strain, HSF1 levels were depleted (left panel) with DOX without altering HSP90 expression (right panel). HSF1 and HSP90 transcript levels were normalized to ACT1 and GPD1. Data are means +/- standard error of the means for triplicate samples. ** indicates P value <0.01, ns indicates no significant difference, unpaired t test. b) HSF1 depletion induces filamentation independently of changes in HSP90 expression.