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. 2018 Mar 28;14(3):e1007270. doi: 10.1371/journal.pgen.1007270

Fig 8. Overexpression of HSF1 induces filamentation through overexpression of positive regulators of filamentation.

Fig 8

a) Overexpression of HSF1 drives the overexpression of UME6 and BRG1. Strains were grown in the presence of no DOX, 0.1 μg/mL DOX (Low DOX), or 20 μg/mL DOX (High DOX) at 30°C. UME6 and BRG1 transcript levels were normalized to ACT1 and GPD1. Data are means +/- standard error of the means for triplicate samples. b) Overexpression of UME6 or BRG1 induces filamentation. Strains with a tetracycline-inducible promoter (tetON) driving the expression of target genes were grown in the presence of 50 μg/mL DOX to induce overexpression at 30°C. Overexpression of LEU3 acts as a negative control. c) Loss of Ume6 or Brg1 blocks filamentation induced by HSF1 overexpression. Strains were grown in the absence of DOX at 30°C.