Figure 4.

Effects of pterostilbene on autophagy-associated signals of CAR cells. Cells were treated with 0, 50 and 75 μM pterostilbene for 24 h, and then total RNA and protein were individually prepared. Reverse transcription-quantitative polymerase chain reaction was performed to measure (A) LC3-II and (B) Atg12 mRNA expression. Values represent the mean ± standard deviation of three independent experiments. ^*P<0.05 vs. untreated control. (C) Representative blots of Atg5, Atg7, Atg12, Beclin-1 and LC3, as detected by western blotting. Representative images were taken from three independent experiments. LC3, light chain 3; Atg, autophagy related gene.