Figure 6.

Effects of pterostilbene on apoptosis and the caspase-dependent signaling of CAR cells. Cells were incubated with 0, 25, 50, 75 or 100 μM pterostilbene for 48 h following pre-incubation with or without 15 μM Z-VAD-FMK for 1 h. (A) TUNEL-positive cells were detected using a commercial kit and analyzed using flow cytometry. (B) Cell viability was measured using the MTT assay. Values represent the mean ± standard deviation of three independent experiments. ^*P<0.05 vs. untreated control; ^#P<0.05 vs. exposure to pterostilbene alone. Z-VAD-FMK, carbobenzoxyvalyl-alanyl-aspartyl fluoromethyl ketone; TUNEL, terminal deoxynucleotidyl transferase-mediated d-UTP nick end labeling.