Figure 7.

Effects of pterostilbene on the mitochondria-dependent apoptotic signaling of CAR cells. Cells were incubated with 0, 25, 50, 75 or 100 μM pterostilbene for 48 h, and whole-cell lysates were then harvested. (A) Caspase-3 and (B) -9 activities were determined using a colorimetric assay. Values represent the mean ± standard deviation of three independent experiments. ^*P<0.05 vs. untreated control. (C) Cell fractions were probed with anti-Bcl-2, anti-Bax, anti-cytochrome c, anti-caspase-9, anti-caspase-3, anti-caspase-7 and anti-PARP antibodies and assessed via western blotting. Representative images were taken from three independent experiments. PARP, Poly (ADP-ribose) polymerase.