Figure 8.

Effects of pterostilbene on MDR1 expression and AKT signaling in CAR cells. Cells were treated with or without 50 and 75 μM pterostilbene for 24 h, harvested and underwent RT-qPCR analysis and western blot analysis, respectively. (A) Total RNA was extracted, and RT-qPCR analysis was performed to determine MDR1 mRNA expression. Values represent the mean ± standard deviation of three independent experiments. ^*P<0.05 vs. untreated control. (B) Western blotting was performed to measure MDR1, p-AKT on Ser473 and total AKT protein expression. Representative images were from three independent experiments. MDR1, multidrug resistance protein 1; AKT, protein kinase B; p-, phosphorylated.