Figure 3. Deficiency of iRhom2 attenuated inflammatory cell infiltration without affecting renal deposition of IC and C3 in the kidneys of Fcgr2b^–/– mice.

(A) Kidneys were stained for mIgG and C3 by immunofluorescence. Photographs shown represent kidneys from n = 4 WT, n = 4 Rhbdf2^–/–, n = 10 Fcgr2b^–/–, and n = 10 Fcgr2b^–/–Rhbdf2^–/– mice. Scale bar: 25 μm. (B–F) Inflammatory cell infiltrates were analyzed by flow cytometry in mouse kidneys. Cell numbers of CD45⁺ leukocytes (B), macrophages (C), neutrophils (D), Ly6C^hi monocytes, (E) and T cell subsets (F) are illustrated. Numbers shown are cells per kidney. (B–E) n = 8 WT, n = 5 Rhbdf2^–/–, 8 Fcgr2b^–/–, n = 8 Fcgr2b^–/–Rhbdf2^–/– mice. (F) n = 10 Fcgr2b^–/–, n = 9 Fcgr2b^–/–Rhbd2^–/– mice. Data are shown as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001, 1-way ANOVA with Dunnett’s multiple comparisons test (A–E), 2-tailed unpaired Student’s t test (F).