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. 2018 Mar 5;128(4):1397–1412. doi: 10.1172/JCI97650

Figure 9. Blockade of EGFR signaling protected Fcgr2b–/– mice from severe kidney injury.

Figure 9

Female Fcgr2b–/– mice were treated with erlotinib (E) or vehicle control (V) (10 mice/group). Kidney damage was assessed by BUN (A), proteinuria (B) and histology (C, PAS and Masson trichrome staining). Scale bars: 50 μm. (D) Serum anti-dsDNA IgGs upon euthanasia between 7 and 9 months. (E) qPCR analysis of renal expression of Ctgf and Col1a1. n = 3 WT, n = 3 Rhbdf2–/–, n = 8 vehicle-treated Fcgr2b–/– mice, n = 8 erlotinib-treated Fcgr2b–/– mice, n = 8 Fcgr2b–/–Rhbdf2–/– mice. (F) Kidney expression of p-EGFR and p-ERK1/2. Total ERK1/2 was used as control. Data are shown as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001, 2-tailed unpaired Student’s t test (A, B, D, F), 2-tailed Mann-Whitney U test (C), 1-way ANOVA with Dunnett’s multiple comparisons test (E).