Figure 2. Gene expression map across data sets examining the effects of RTX on gene expression.

Data from 7 experiments were mined and analyzed, including 3 experiments in which the TRPV1 agonist RTX was used to selectively damage TRPV1⁺ neurons. Genes decreasing by at least 40% are plotted for the 3 experiments in which RTX was used to damage TRPV1⁺ neurons, and compared with several data sets showing differentials between tissues or cell types to indicate where these genes are expressed. In general, these genes are contributed by neurons, as indicated by their enrichment in DRG versus sciatic nerve, and sorted eTRPV1 neurons versus eIB4⁺ cells, which includes non-neuronal cells such as microglia and vascular endothelia. Enrichment in TRPV1⁺ neurons in the single-cell data set was tabulated separately. Modest enrichment in these cells was observed in the rat and dog but not human data sets. Values were normalized such that the highest value in any data set is 1. Data from single cells are normalized differently, according to their original publication. Quantification of enrichment in TRPV1⁺ and nonpeptidergic 1 populations of cells versus other groups is presented. Genes from rat and dog samples showing a decrease after RTX treatment are enriched in the TRPV1⁺ cells.