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. 2018 Mar 16;52(5):1415–1426. doi: 10.3892/ijo.2018.4324

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Copyright: © Du et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Evaluation of BRD4 expression in HA and glioma U251 cells. (A) mRNA expression levels of BRD4 in glioma U251 cells and normal HA cells. (B) Western blot analysis of the protein expression levels of BRD4 in U251 cells and normal HA cells. (C) mRNA levels and (D) protein expression levels of BRD4 were effectively inhibited by BRD4-shRNAs. GAPDH was used as an internal control. Data are presented as the mean ± standard deviation of three independent experiments. ^*P<0.05 and ^**P<0.01, with comparisons indicated by lines. BRD4, bromodomain containing 4; HA, human astrocytes; sh, short hairpin; Scr, scrambled control.