Table I.
Primers used in quantitative polymerase chain reaction analysis.
| Gene | Sequence (5′–3′) |
|---|---|
| KRAS | F: CAGTAGACACAAAACAGGCTCAG |
| R: TGTCGGATCTCCCTCACCAATG | |
| BRAF | F: AACGAGACCGATCCTCATCAGC |
| R: GGTAGCAGACAAACCTGTGGTTG | |
| CALM2 | F: AGTGCTGCAGAACTTCGCCATG |
| R: CAAGGTCTTCACTTTGCTGTCATC | |
| ARAF | F: CCCACATTCCAAGTCACCAGCA |
| R: CCTCCCAGTAATAGCCTGAGTC | |
| RHOA | F: TCTGTCCCAACGTGCCCATCAT |
| R: CTGCCTTCTTCAGGTTTCACCG | |
| MAPK8 (JNK1) | F: GACGCCTTATGTAGTGACTCGC |
| R: TCCTGGAAAGAGGATTTTGTGGC | |
| PLCB3 | F: GGTGAATGCCACTGAGGAGATG |
| R: CTTGGTCTCCACAAAGGACGAC | |
| MAPK10 (JNK3) | F: GTGTGGAAGTGGGAGACTCAAC |
| R: GTCAAGGACAGCATCATACGCG | |
| GNAI1 | F: AGCACTGAGTGACTACGACCTG |
| R: GGATGTATCTGTAAACCACTTGTTG | |
| ADCY6 | F: CACATAGCACCGCAGTTGGCAT |
| R: AGGCAGTGATGTCAGCAGGTGT | |
| GAPDH | F: CCGGGAAACTGTGGCGTGATGG |
| R: AGGTGGAGGAGTGGGTGTCGCTGTT |
KRAS, KRAS proto-oncogene GTPase; BRAF, B-Raf proto-oncogene serine/threonine kinase; CALM2, calmodulin 2; ARAF, A-Raf proto-oncogene serine/threonine kinase; RHOA, ras homolog family member A; MAPK, mitogen-activated protein kinase; JNK, c-Jun N-terminal kinase; PLCB3, phospholipase C β3; GNAI1, G protein subunit α i1; ADCY6, adenylate cyclase 6; F, forward; R, reverse.