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. 2018 Mar 5;52(5):1515–1527. doi: 10.3892/ijo.2018.4304

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Effects of SIRT6 on colon cancer cell lines. (A) Expression of SIRT6 in different colon cancer cell lines. The RKO cell line had the highest expression and the HCT116 cell line had the lowest expression. (B) mRNA expression levels of SIRT6 after silencing or overexpression were evaluated by quantitative polymerase chain reaction to confirm the efficiency of transfection. si-SIRT6 markedly downregulated the expression of SIRT6 mRNA in RKO cells, and the SIRT6 overexpression plasmid vector increased the expression of SIRT6 in HCT116 cells. (C) Expression of SIRT6 after silencing or overexpression was assessed by western blot analysis, to confirm the efficiency of transfection. si-SIRT6 markedly downregulated the expression of SIRT6 in RKO cells, and the SIRT6 overexpression plasmid vector increased the expression of SIRT6 in HCT116 cells. (D) SIRT6 overexpression inhibited cell growth compared with in the control groups, whereas silencing SIRT6 promoted cell proliferation compared with in the control groups; ^*P<0.05 vs. the NC, or control or blank groups. (E and F) Annexin V-FITC/PI was used to detect apoptosis after silencing SIRT6 in RKO cells or overexpressing SIRT6 in HCT116 cells. Cell apoptosis was elevated in the SIRT6 group and decreased in the si-SIRT6 group compared with in the control groups; ^*P<0.05 vs. the NC-siRNA, control or blank groups. (G and H) Colony formation assays indicated that silencing SIRT6 enhanced the colony-forming ability of cells, whereas overexpression of SIRT6 exhibited the opposite effect; ^*P<0.05 vs. the NC-siRNA, control or blank groups. FITC, fluorescein isothiocyanate; NC, negative control; OD, optical density; PI, propidium iodide; si/siRNA, small interfering RNA; SIRT6, sirtuin 6.

Effects of SIRT6 on colon cancer cell lines. (A) Expression of SIRT6 in different colon cancer cell lines. The RKO cell line had the highest expression and the HCT116 cell line had the lowest expression. (B) mRNA expression levels of SIRT6 after silencing or overexpression were evaluated by quantitative polymerase chain reaction to confirm the efficiency of transfection. si-SIRT6 markedly downregulated the expression of SIRT6 mRNA in RKO cells, and the SIRT6 overexpression plasmid vector increased the expression of SIRT6 in HCT116 cells. (C) Expression of SIRT6 after silencing or overexpression was assessed by western blot analysis, to confirm the efficiency of transfection. si-SIRT6 markedly downregulated the expression of SIRT6 in RKO cells, and the SIRT6 overexpression plasmid vector increased the expression of SIRT6 in HCT116 cells. (D) SIRT6 overexpression inhibited cell growth compared with in the control groups, whereas silencing SIRT6 promoted cell proliferation compared with in the control groups; ^*P<0.05 vs. the NC, or control or blank groups. (E and F) Annexin V-FITC/PI was used to detect apoptosis after silencing SIRT6 in RKO cells or overexpressing SIRT6 in HCT116 cells. Cell apoptosis was elevated in the SIRT6 group and decreased in the si-SIRT6 group compared with in the control groups; ^*P<0.05 vs. the NC-siRNA, control or blank groups. (G and H) Colony formation assays indicated that silencing SIRT6 enhanced the colony-forming ability of cells, whereas overexpression of SIRT6 exhibited the opposite effect; ^*P<0.05 vs. the NC-siRNA, control or blank groups. FITC, fluorescein isothiocyanate; NC, negative control; OD, optical density; PI, propidium iodide; si/siRNA, small interfering RNA; SIRT6, sirtuin 6.