Figure 6.

(A) Transfection efficiency was confirmed by quantitative polymerase chain reaction. miR-34c-5p mimics upregulated the expression of miR-34c-5p in RKO cells and miR-34c-5p inhibitors downregulated the expression of miR-34c-5p in HCT116 cells. (B) Effects of miRNA mimics and inhibitors on SIRT6 expression in colon cancer cell lines, as determined by western blot analysis. SIRT6 expression was suppressed by miR-34c-5p mimics and increased by inhibitors. (C and D) Dual-luciferase reporter assays used vectors encoding wt or mut putative miR-34c-5p target sites in the SIRT6 3′-UTR. Data were normalized by Renilla/Firefly luciferase activity. The relative luciferase activity of the mimics + wt group was clearly lower, and that of the inhibitors + wt group was significantly higher compared with the other groups; ^*P<0.05 vs. other groups. con, control vector; in, inhibitors; mi, mimics; miR, microRNA; mut, mutant; NC, negative control; SIRT6, sirtuin 6.