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. 2018 Mar 5;52(5):1515–1527. doi: 10.3892/ijo.2018.4304

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Effects of miR-34c-5p on colon cancer cells. (A) Cell viability was determined using Cell Counting kit-8 assays post-transfection with mimics or inhibitors in RKO or HCT116 cell lines. miR-34c-5p mimics facilitated cell growth compared with in the control groups, whereas miR-34c-5p inhibitors suppressed cell viability; ^*P<0.05 vs. other groups. (B and C) Annexin V-FITC/PI was employed to estimate apoptosis post-transfection with miR-34c-5p mimics in RKO cells or miR-34c-5p inhibitors in HCT116 cells. The results indicated that cell apoptotic rate was decreased in the mimic group and increased in the inhibitor group compared with in the control groups; ^*P<0.05 vs. other groups. (D and E) Compared with in the other groups, transfection with miR-34c-5p inhibitors inhibited colony formation, whereas miR-34c-5p mimics enhanced it; ^*P<0.05 vs. other groups. (F) JAK2 and STAT3 phosphorylation was promoted by mimics and decreased by inhibitors compared with in the other groups. miR-34c-5p may promote cell growth and inhibit apoptosis via the JAK2/STAT3 pathways. FITC, fluorescein isothiocyanate; JAK2, Janus kinase 2; miR, microRNA; NC, negative control; OD, optical density; p-, phosphorylated; PI, propidium iodide; SIRT6, sirtuin 6; STAT3, signal transducer and activator of transcription 3; t, total.

Effects of miR-34c-5p on colon cancer cells. (A) Cell viability was determined using Cell Counting kit-8 assays post-transfection with mimics or inhibitors in RKO or HCT116 cell lines. miR-34c-5p mimics facilitated cell growth compared with in the control groups, whereas miR-34c-5p inhibitors suppressed cell viability; ^*P<0.05 vs. other groups. (B and C) Annexin V-FITC/PI was employed to estimate apoptosis post-transfection with miR-34c-5p mimics in RKO cells or miR-34c-5p inhibitors in HCT116 cells. The results indicated that cell apoptotic rate was decreased in the mimic group and increased in the inhibitor group compared with in the control groups; ^*P<0.05 vs. other groups. (D and E) Compared with in the other groups, transfection with miR-34c-5p inhibitors inhibited colony formation, whereas miR-34c-5p mimics enhanced it; ^*P<0.05 vs. other groups. (F) JAK2 and STAT3 phosphorylation was promoted by mimics and decreased by inhibitors compared with in the other groups. miR-34c-5p may promote cell growth and inhibit apoptosis via the JAK2/STAT3 pathways. FITC, fluorescein isothiocyanate; JAK2, Janus kinase 2; miR, microRNA; NC, negative control; OD, optical density; p-, phosphorylated; PI, propidium iodide; SIRT6, sirtuin 6; STAT3, signal transducer and activator of transcription 3; t, total.