(A) Hierarchical clustering of median-centered RNA gene expression defined
as significantly differentially expressed genes in metastases (Mets) as compared with
matched primary tumors (Primaries) with the lmer function in R. Each
color in the dendrogram identifies a different patient. Box plots of the mean signature
score of (B) upregulated genes and (C) downregulated genes,
comparing the following categories: TCGA normal breast tissue, TCGA luminal A/B primary
tumors, UNC RAP luminal primary tumors, UNC RAP luminal metastases, TCGA HER2-enriched
primary tumors, UNC RAP HER2-enriched primary tumors, UNC RAP HER2-enriched metastases,
TCGA basal-like primary tumors, UNC RAP basal-like primary tumors, and UNC RAP
basal-like metastases. ADR, adrenal gland; Ax-LN, axillary lymph node; Basal,
basal-like; HER2E, HER2-enriched; Lum, luminal; LLL, left lower lobe; LUL, left upper
lobe; MED, mediastinum MET, metastases; OVA, ovary; PRIM, primary tumor; RLL, right
lower lobe; SKIN-L, left skin; SKIN-R, right skin ; SPIN, spinal.