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. 2018 Mar 5;128(4):1471–1484. doi: 10.1172/JCI97488

Figure 5. Tie2 deficiency alone recapitulates sepsis-associated thrombotic response.

Figure 5

(A–E) Platelet and fibrin accumulation was monitored in Tie2 heterozygous mice (Tie2+/–) and wild-type littermate controls (Tie2+/+) at the site of laser injury using anti-platelet Dylight 649–labeled and anti-fibrin Dylight 488–labeled antibodies. (A) Representative binarized images of a single thrombus (platelet = red; fibrin = green) at time of laser injury (0 seconds) and 30 to 120 seconds following laser injury. Median integrated fluorescence intensities and AUC were calculated for platelets (B and C) and fibrin (D and E). (C and E) Data are represented as the median for individual thrombi (Tie2+/–, n = 41; Tie2+/+, n = 40). (F–J) Platelet and fibrin generation was monitored in wild-type mice infused with eptifibatide (10 μg/g body weight) and exposed to saline or 10 mg/kg LPS. (F) Representative binarized images of thrombus formation, at time of laser injury (0 seconds) and up to 120 seconds following laser injury. Median integrated fluorescence intensities were calculated for platelets (G) and fibrin (I). (H and J) Data are represented as median individual thrombi (saline, n = 35; LPS n = 32). **P < 0.01, Mann-Whitney U test. Scale bars: 10 μm.