Table 2. Prediction of essential pathways in Acetobacter fabarum DsW_054.
| KO | Pathway | No insertions | All genes | p-value | fdr corrected p-value | Predicted essentiality |
|---|---|---|---|---|---|---|
| ko02010 | ABC transporters | 11 | 236 | 0.0005 | 0.01 | nonessential |
| ko03010 | Ribosome | 46 | 52 | 0.0005 | 0.01 | essential |
| ko00970 | Aminoacyl-tRNA biosynthesis | 22 | 24 | 0.0005 | 0.01 | essential |
| ko03060 | Protein export | 13 | 19 | 0.0005 | 0.01 | essential |
| ko04112 | Cell cycle - Caulobacter | 12 | 14 | 0.0005 | 0.01 | essential |
| ko00500 | Starch and sucrose metabolism | 1 | 75 | 0.0015 | 0.03 | nonessential |
| ko02020 | Two-component system | 15 | 170 | 0.0025 | 0.04 | nonessential |
| ko00195 | Photosynthesis | 7 | 7 | 0.0035 | 0.05 | essential |
| ko01120 | Microbial metabolism in diverse environments | 47 | 376 | 0.0040 | 0.05 | nonessential |
| ko00330 | Arginine and proline metabolism | 1 | 53 | 0.0055 | 0.06 | nonessential |
| ko00010 | Glycolysis / Gluconeogenesis | 3 | 66 | 0.0085 | 0.08 | nonessential |
| ko00240 | Pyrimidine metabolism | 28 | 78 | 0.0110 | 0.09 | essential |
| ko02040 | Flagellar assembly | 1 | 42 | 0.0110 | 0.09 | nonessential |
| ko00780 | Biotin metabolism | 11 | 22 | 0.0115 | 0.09 | essential |
| ko01502 | Vancomycin resistance | 5 | 5 | 0.0180 | 0.13 | essential |
| ko01110 | Biosynthesis of secondary metabolites | 107 | 419 | 0.0200 | 0.13 | essential |
| ko00052 | Galactose metabolism | 1 | 41 | 0.0210 | 0.130 | nonessential |
| ko00620 | Pyruvate metabolism | 4 | 69 | 0.0235 | 0.14 | nonessential |
| ko00550 | Peptidoglycan biosynthesis | 11 | 25 | 0.0360 | 0.20 | essential |
| ko01230 | Biosynthesis of amino acids | 49 | 176 | 0.0380 | 0.20 | essential |
| ko00250 | Alanine, aspartate and glutamate metabolism | 13 | 34 | 0.0425 | 0.20 | essential |
| ko00561 | Glycerolipid metabolism | 1 | 31 | 0.0425 | 0.20 | nonessential |
| ko00740 | Riboflavin metabolism | 6 | 10 | 0.0435 | 0.20 | essential |
| ko02024 | Quorum sensing | 12 | 112 | 0.0480 | 0.21 | nonessential |
| ko00400 | Phenylalanine, tyrosine and tryptophan biosynthesis | 13 | 33 | 0.0500 | 0.21 | essential |
Genes with no insertions were grouped into functional pathways using KEGG pathway mapper. The ‘no insertions’ column shows the number of genes that have no insertions within our library for each pathway. This was compared to the number of genes within that pathway that are present in A. fabarum (‘All genes’ column) using a chi-square test and the associated p-value and fdr corrected p-value are listed in addition to the predicted essentiality status for each pathway.