Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| genetic reagent (D. melanogaster) |
dnlg1ex1.9 | PMID: 20547130 | ||
| genetic reagent (D. melanogaster) |
dnlg1ex2.3 | PMID: 20547130 | ||
| genetic reagent (D. melanogaster) |
dnlg2KO70 | PMID: 21228178 | ||
| genetic reagent (D. melanogaster) |
dnlg2KO17 | PMID: 21228178 | ||
| genetic reagent (D. melanogaster) |
dnrx83 | PMID: 23352167; PMID: 17498701 | ||
| genetic reagent (D. melanogaster) |
dnrx174 | PMID: 23352167; PMID: 17498701 | ||
| genetic reagent (D. melanogaster) |
UAS-DNrx | PMID: 23352167; PMID: 17498701 | ||
| genetic reagent (D. melanogaster) |
UAS-SCAR RNAi | Center of Biomedical Analysis, Tsinghua University | TH: 02179 .N; RRID: BDSC_51803 | |
| genetic reagent (D. melanogaster) |
UAS-DNlg1 | lab generated; this paper | ||
| genetic reagent (D. melanogaster) |
UAS-DNlg1SF-AA | lab generated; this paper | ||
| genetic reagent (D. melanogaster) |
UAS-DNlg1-EGFP | lab generated; this paper | ||
| genetic reagent (D. melanogaster) |
UAS-DNlg1SF-AA-EGFP | lab generated; this paper | ||
| genetic reagent (D. melanogaster) |
OK6-Gal4 | Bloomington Drosophila Stock center | BDSC: 64199 | |
| genetic reagent (D. melanogaster) |
Elav-Gal4 | Bloomington Drosophila Stock center | BDSC: 458 | |
| genetic reagent (D. melanogaster) |
C57-Gal4 | PMID: 8893021 | ||
| genetic reagent (D. melanogaster) |
24B-Gal4 | Kyoto Stock center | DGGR:106496 | |
| genetic reagent (D. melanogaster) |
Mef2-Gal4 | Bloomington Drosophila Stock center | BDSC: 27390 | |
| cell line (D. melanogaster) |
S2 | China Center for Type Culture Collection, CCTCC | GDC138; RRID:CVCL_Z992 | Improved STR profiling Mycoplasma contamination test: negative |
| antibody | Mouse anti-α-Spectrin | Developmental Studies Hybridoma Bank | DSHB: 3A9; RRID: AB_528473 | 1:50 for IHC |
| antibody | anti-DLG | Developmental Studies Hybridoma Bank | DSHB:4F3; RRID: AB_528203 | 1:50 for IHC |
| antibody | anti-Brp | Developmental Studies Hybridoma Bank | DSHB:nc82;RRID:AB_2314866 | 1:50 for IHC |
| antibody | anti-SCAR | Developmental Studies Hybridoma Bank | DSHB: P1C1-SCAR; RRID:AB_2618386 | 1:50 for IHC, 1:200 forwestern blotting |
| antibody | rabbit anti-HRP | Jackson ImmunoResearch | Jackson ImmunoResearch: 323-005-021 RRID: AB_2314648 | 1:1000 for IHC |
| antibody | Goat anti-HRP | Jackson ImmunoResearch | Jackson ImmunoResearch: 123-005-021 RRID: AB_2338952 | 1:1000 for IHC |
| antibody | Mouse anti-Tubulin | Sigma | Sigma: Clone DM1A; RRID:AB_477593 | 1:10000 for western blotting |
| antibody | Alexa 488-, 555-, or 637- secondaries | Molecular Probes | ||
| Other | Texas Red-conjugated Phalloidin | Molecular Probes | ThermoFisher: T7471 | 1:6 for IHC |