TABLE 1.
Antigen | Immunogen | Manufacturer | Dilution |
---|---|---|---|
Choline acetyltransferase | Human placental ChAT | EMD Millipore (Billerica, MA), goat polyclonal, AB144P RRID: AB_2079751 |
1:200 |
Tyrosine hydroxylase | Denatured rat TH | EMD Millipore, rabbit polyclonal, AB152 RRID: AB_390204 |
1:2,000 |
Tryptophan hydroxylase 2 | KLH-conjugated peptide derived from human TPH2 (proprietary) | EMD Millipore, rabbit polyclonal, ABN60 RRID: AB_10806898 |
1:500 |
Somatostatin-14 | Synthetic peptide | Peninsula Laboratories (San Carlos, CA), rabbit polyclonal, T-4103 RRID: AB_518614 |
1:1,000 |
Cholera toxin subunit B | Purified toxin subunit (Choleragenoid) | List Biological Labs (Campbell, CA), goat polyclonal, #703 RRID: AB_10013220 |
1:1,000 |
Cholera toxin subunit B | Purified Choleragenoid | Abcam (Cambridge, MA), rabbit polyclonal, ab34992 RRID: AB_726859 |
1:2,000 |
Substance P | Substance P conjugated to BSA | EMD Millipore, rat monoclonal, MAB356 RRID: AB_94639 |
1:400; 1:2,000 floating |