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. 2018 Mar 28;13(3):e0193583. doi: 10.1371/journal.pone.0193583

Fig 3. Increased immunohistochemical staining of HN in IUGR-affected placentas.

Fig 3

(A) Immunohistochemical staining of humanin (HN) in human placental tissue at gestational age of 38 weeks. Humanin was stained in brown. The left column shows staining of the stem villi EVT of placental tissue, and the right column shows staining of the maternal surface EVT of placental tissue. The top row of both columns is negative control performed with non-isotype IgG. The middle row of both columns is placenta from normal pregnancies. The bottom row of both columns is IUGR. 40x magnification for all images. (B) Immunohistochemical staining in normal (control) human placental tissue showing four different markers of same placental sample (serial sections) at 38 weeks gestational age. The top row is the non-isotype IgG control for each of the markers labeled. The bottom row from left to right is staining of AE1/AE3 (trophoblast marker), GLUT8, Humanin, and lysozyme (lysosomal marker). The presence of EVT on the right portion of each image is supported by diffuse cytoplasmic staining of AE1/AE3, while the endometrial stromal cells within the decidua on the left portion of each image are negative for AE1/AE3. Each of the four images on the bottom row shows the decidua on the left side and maternal surface EVT on the right side of the image. Arrows point to maternal surface EVT and triangles indicate decidual stromal cells. All images were taken at 20x magnification.