Fig 4. Immunoprecipitation (IP) assays reveal association between endogenous humanin with Glut8 in HTR-8 cells.

(A) HTR8/SVneo protein was immunoprecipitated with Glut8 antibody then the bound protein was analyzed by western blot with humanin antibody. (B) HTR8/SVneo protein lysate was immunoprecipitated with humanin antibody then analyzed by western blot with glut8 antibody. In both the cases Rabbit non-isotype IgG was used as negative control (-C) and in positive control (+C), both the immunoprecipitation and western blot analyses were done using the same antibody (either humanin or Glut8 antibody).