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. 2018 Mar 28;13(3):e0193779. doi: 10.1371/journal.pone.0193779

Fig 9. Effect of siRNA-mediated knockdown of NKP on pII cell motility.

Fig 9

Panel A: NKP mRNA expression in pII cells transfected with a scrambled sequence (control, open bar) or NKP siRNA (48 h, solid bar). RNA was extracted from cells, converted to cDNA and amplified by PCR. Ct values were converted to ratios relative to actin. Panel B: mean distance moved (in pixels) as a measurement of cell motility for pII cell transfected with a scrambled sequence (control, open bar, taken as 100%) or NKP siRNA (48 h, solid bar) determined after 24 h of incubation. Histobars represent means ± SEM of at least 3 independent determinations. Asterisk denotes significant difference from control with p < 0.05. Panel C: The photographs represent an example of motility for each treatment condition.