Fig 2. Measurement of ZIKV specific and DENV cross-reactive antibody responses elicited by ZIKV E80_S and E80_E proteins.

(A) and (B) Serum samples from different groups of mice (n = 5 for each group) were 2-fold serially diluted from 1:320 to 1:40960 and measured for (A) end-point dilution titers and (B) OD₄₅₀ values by using ZIKV E80 protein coated ELISA. (C) and (D) Serum sample obtained from mice received PBS (n = 5), 50μg E80_E (n = 4) and 50μg E80_S (n = 5) were 2-fold serially diluted from 1:160 to 1:20480 and measured for (C) end-point dilution titers and (D) OD₄₅₀ values by using DENV-3 E80 protein coated ELISA. The statistical differences between PBS control and immunization groups were determined by Student’s t test, and marked with black stars. A p<0.05 value was designated as *, p<0.01 as **, and p<0.001 as ***. Red stars indicate statistical differences between the 10μg E80_E group and other immunization groups. The data were presented as mean ± SEM. (A) and (B) Each sample was assayed in duplicates and the figures are representative results of 4 independent experiments. (C) and (D) Each sample was assayed in duplicates and the figures are representative results of 2 independent experiments.