Fig 3. Assessment of serum neutralization activity by plaque reduction neutralization test (PRNT).

Serum samples were 3-fold serially diluted from 1:10 to 1:2430, and mixed with an equal volume of medium containing 100 PFU Zika virus then applied on Vero cells. Neutralizing activity was calculated as described in the Materials and Methods. (A) Summary of PRNT₅₀ titer of each mouse in different experimental groups. Red stars represent significant differences (Student’s t tests) between the PBS control group and the indicated vaccination group against serum dilutions. (C) Two-way ANOVA test, followed by Tukey's correction for multiple comparisons were used to determine statistical differences among curves plotted in (B). The data were presented as mean ± SEM. Each sample was assayed in duplicates and the figures are representative results of 4 independent experiments.