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. 2018 Mar 28;13(3):e0194860. doi: 10.1371/journal.pone.0194860

Fig 5. Protective efficacy of vaccines against ZIKV viremia.

Fig 5

Viremia levels of immunized mice after ZIKV challenge were presented by percentage of infected Vero cells. 10μl of serum samples obtained at various days post infection were applied to Vero cells in DMEM containing 1% FBS for 4 days. The percentage of infected cells was determined by staining with an anti-E antibody 4G2, followed by an anti-mouse AF488 conjugated antibody, and then analyzed by flow cytometry. (A) 10μg E80_E group (n = 4), (B) 50μg E80_E group (n = 4), (C) PBS group (n = 5), (D) 10μg E80_S group (n = 5), and (E) 50μg E80_S group (n = 5). Each line represented one mouse. (F) Area under the curve (AUC) was calculated by Graphpad Prism 6 software. (G) Maximum percentage of infected cells from 1 to 5 DPI for each experimental group. (H) Duration of detectable viremia. (I) Summary of viremia data for each of the groups at days 1 to 5 post infection. The data were presented as mean ± SEM. Statistical differences were determined by Student’s t test and p values were indicated by * (p<0.05), or ** (p<0.01), or *** (p<0.001). The figures are representative results of 2 independent experiments.