Table 2. Efficiency of the miRNA cassette subcloning into pKK-RNAi vectors.
Constructs attempted | First attempt successful (fraction) |
Correct sequence on first clone checked (fraction) |
---|---|---|
50 | 50 (100%) |
50 (100%) |
Constructs attempted | First attempt successful (fraction) |
Correct sequence on first clone checked (fraction) |
---|---|---|
50 | 50 (100%) |
50 (100%) |