| Primary Antibody Antigen |
Immunogen |
Manufacturer |
Dilution used |
| TRPV1 |
C-terminus of rat TRPV1 (824–838) |
Alomone labs, Jerusalem, Israel; rabbit polyclonal; # ACC030 |
1:1000 |
| TRPM8 |
C-terminus (amino acids 1005–1104) of rat TRPM8 |
Supplied by Hiroshi Hosokawa; rabbit polyclonal [20] |
1:5000 |
| β-Tubulin III |
Microtubules from rat brain |
Covance, Emeryville, CA; mouse monoclonal; Cat # MMS-435P |
1:2000 |
| Secondary antibody |
Manufacturer |
Dilution used |
| Donkey α rabbit Alexa594 |
Molecular probes, Invitrogen; #A21207 |
1:200 |
| Donkey α mouse Alexa488 |
Molecular probes, Invitrogen; #A11001 |
1:200 |
