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. 2018 Mar 28;13(3):e0195054. doi: 10.1371/journal.pone.0195054

Fig 6. 5S rDNA and U2 snDNA dual-colour FISH in selected botiid species.

Fig 6

U2 snDNA (red, arrows) and 5S rDNA (green, arrowheads) probes mapped on mitotic chromosomes of (A) B. udomritthiruji, (B) L. microphthalma, (C) Y. lecontei. Chromosomes were counterstained with DAPI (blue). Note the significant spreading of 5S rDNA sites in Y. lecontei (C; 24 signals—arrowheads). The metaphase spread of B. udomritthiruji displays incomplete chromosome set (2n = 97), however the number of two U2 snDNA-bearing chromosomes was consistently observed on all other (including complete though less representative) metaphases in our dataset and with respect to Fig 5B, the number of 5S rDNA-bearing chromosomes is also complete. Bar = 10 μm.