Fig. 4. In-silico search for CDR3β aminoacid sequences from ZnT8_186–194-reactive CD8⁺ T-cell clones.

(A–C) Prevalence of the CDR3β aminoacid sequences from clones D010R 1E2 (A), H328C 8E8 (B) and H034O 141B9 (C) among HLA-A2⁺ T1D (n=5), aAb⁺ (n=5) and healthy subjects (n=10), as assessed by in-silico analysis of TCRβ repertoires obtained from the indicated CD8⁺ and CD4⁺ T-cell subsets. (D) In-silico search for the same CDR3β aminoacid sequences in the repertoire of CD8⁺, conventional CD4⁺ (Tconv; CD127⁺) and regulatory CD4⁺ (Treg; CD25⁺CD127⁻) T cells obtained from nPOD PLN, spleen and inguinal lymph node (ILN) samples via the online database http://clonesearch.jdrfnpod.org. For each cell type and tissue, the first, second and third columns refer to clones D010R 1E2, H034O 141B9 and H328C 8E8, respectively. Dark and light grey cells indicate negative and positive samples, respectively. Frequencies per 10⁶ TCRs are annotated, and underlining indicates samples with a nucleotide sequence match. White cells indicate unavailable samples. Pancreatic NET, neuro-endocrine tumor.