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a SDS-PAGE and b zymogram of α-amylase. Lane 1: SDS-PAGE of ammonium sulfate (80%) precipitated protein; Lane 2: SDS-PAGE of purified α-amylase after ion-exchange chromatography (54 kDa); Lane 3: Zymogram of purified α-amylase. L: EZ-Run Prestained Rec Protein Ladder
