Table 1.
Purification step | Total activity (U) | Total protein (mg) | Specific activity (U/mg) | Purification (fold) | Yield (%) |
---|---|---|---|---|---|
Crude culture | 86.10 | 4.560 | 18.88 | 1.0 | 100.0 |
80% (NH4)2SO4 precipitation | 26.42 | 0.759 | 34.81 | 1.84 | 30.69 |
Anion-exchange chromatography | 10.1 | 0.210 | 48.10 | 2.55 | 11.73 |