Table 3.

Competency of in planta transformation methodology on different peanut cultivars

Cultivars used for transformation	No. of seeds infected	Mean no. of germinated seeds on selection mediuma	Mean no. of plants surviving after BASTA® sprayb	Mean no. of plants expressing gus A gene	Transformation efficiency (%)
CO7	100	44.3 ± 0.26d	35.6 ± 0.32d	31.3 ± 0.24e	31.3 ± 0.24e
CO6	100	47.0 ± 0.21b	38.3 ± 0.22c	33.6 ± 0.28d	33.6 ± 0.28d
TMV2	100	45.6 ± 0.25ca	40.0 ± 0.25b	36.3 ± 0.21b	36.3 ± 0.21b
TMV7	100	49.3 ± 0.30a	44.3 ± 0.26a	38.6 ± 0.25a	38.6 ± 0.25a
VR13	100	46.0 ± 0.21c	39.6 ± 0.38ba	35.0 ± 0.25c	35.0 ± 0.25c

The explants from diverse peanut cultivars were pre-cultured for 2 days, sonicated for 6 min, and then vacuum infiltrated for 3 min in A. tumefaciens EHA105 harboring pCAMBIA1301–bar plasmid suspension. The infected explants were incubated for 3 days on MS medium containing 150 µM acetosyringone

Transformation efficiency = no. of GUS-positive plants/no. of infected seeds × 100. Mean values of three separate trials (±) with standard errors. In each column, numbers with different letters indicate they are considerably different from each other according to Duncan’s multiple range test at a probability level of 5%

^aInfected explants were propagated in MS medium supplemented with 4 mg l⁻¹ BASTA^®

^b40-day-old plants were sprinkled with 250 mg l⁻¹ BASTA^®. Visual observations and the data were documented after 1 week