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. 2018 Mar 29;7:49. doi: 10.1038/s41426-018-0042-0

Fig. 4. Sequence alignment and structural comparison of four distinct Hcp types.

Fig. 4

a Sequence alignment and secondary structure of ExPEC Hcp proteins with other known structural homologs. The alignment of Hcp amino-acid sequences was obtained using ClustalW. b The phylogenetic tree and types of 11 Hcp family members selected from Fig. 1a. c The Hcp hexamer of Hcp3 from Pseudomonas aeruginosa (PDB code: 3he1). d Structural comparison of four distinct Hcp types. Hcp-Bp (PDB code: 4w64) is referred to as type Ia, Hcp1-EAEC (PDB code: 4hkh) is referred to as type Ib, and Hcp3-Pa (PDB code: 3he1) is referred to as type IIb. The structural model of Hcp2A (from ExPEC) was drawn based on the template 3he1 (sequence identity, 43.05%) using the SWISS-MODEL server and is referred to as type IIa. The L1, 2 (Ala19 to Ser27) and L2, 3 (Ser56 to His66) loops were designated Variant sequence 1 (Vs1) and Vs2 regions, respectively