Table 3.
Mean of Ct values obtained for the qPCR-based CocciEnv assay
| Site | Replicate 1 | Replicate 2 | Replicate 3 | Replicate 4 | |
|---|---|---|---|---|---|
| 1 | n.d. | n.d. | 39.170457 | n.d. | Low positive |
| 2 | 32.44438067 | 33.684223 | 31.253591 | 32.57464933 | High positive |
| 3 | 38.0875965 | n.d. | n.d. | n.d. | Low positive |
| 4 | 31.043169 | 29.483968 | 31.108808 | 32.5819565 | High positive |
| 5 | n.d. | 37.40752733 | 37.943037 | n.d. | Moderate positive |
| 6 | 32.039917 | 30.76345 | 31.93577333 | 31.11106433 | High positive |
| 7 | n.d. | 38.681686 | n.d. | n.d. | Low positive |
| 8 | 31.93436033 | 30.68055833 | n.d. | n.d. | Moderate positive |
| 9 | 40.3225925 | n.d. | n.d. | 36.743083 | Moderate positive |
| 10 | n.d. | n.d. | n.d. | 39.8178845 | Low positive |
| 11 | n.d. | n.d. | n.d. | 39.017355 | Low positive |
| 12 | 39.985367 | n.d. | 38.475866 | 36.31935467 | Moderate positive |
| 13 | n.d. | 37.238985 | n.d. | n.d. | Low positive |
| 14 | 39.1133525 | n.d. | n.d. | n.d. | Low positive |
| 15 | 36.5966605 | 33.26852667 | n.d. | 37.1464845 | Moderate positive |
DNA extractions were performed in quadruplicates for each site analyzed due to the likelihood of variable and potentially rare presence of Coccidioides DNA in the Lara and Falcon states of Venezuela. Ct values are averages of three replicate reactions for each DNA sample. n.d. not detected