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. 2018 Mar 29;7(3):33. doi: 10.1038/s41389-018-0041-y

Fig. 3. Induction of apoptosis upon combined p53 activation with PKC inhibition.

Fig. 3

a Cell lines OMM2.3, OMM2.5 and OMM1 were treated with 8 µM Nutlin-3 and 4 µM Sotrastaurin. MEL290 was incubated with 2 µM Nutlin-3 and 4 µM Sotrastaurin, cell line MM28 with 8 µM Nutlin-3 and 1 µM Sotrastaurin. and cell lines MEL202, MEL270 and MM66 with 2 µM Nutlin-3 and 0.5 µM Sotrastaurin. All cell lines were incubated for 72 h before harvesting. Protein lysates were analyzed for the expression levels of cleaved and full-length PARP by western blot. Expression of vinculin was analyzed to control for equal loading. b MM66 cells were incubated with 2 µM Nutlin-3 and 0.5 µM Sotrastaurin for 72 h after which the cell cycle profiles were determined by flow cytometry after PI staining, showing an increase in the subG1 fraction upon combined treatment