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. 2018 Mar 28;6:8. doi: 10.1038/s41413-018-0011-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — PU.1 and its co-partner MITF regulate the expression of a network of TFs necessary for OC differentiation. a Graphical representation of the distribution of MITF OC ChIP-Seq peaks throughout the genome. b Treeview plot of genome-wide PU.1 MP and PU.1 OC ChIP-Seq tags ± 3 000 base pairs from MITF MP or MITF OC peak centers. c Venn diagram depicting the OC ChIP-Seq peak sites shared between PU.1 and MITF. d GSEA plot of a TF gene set significantly enriched in genes with overlapping PU.1 and MITF OC ChIP-Seq peaks using our murine MP and OC microarray data (n = 3). Heatmap (right) indicating MP and OC expression of genes in the gene list. e RT-qPCR analysis of 3 TFs on the GSEA gene list, which are necessary for OC differentiation. Gene expression is shown for WT MPs and OCs and Pu.1 KO OCs (n = 3). f RT-qPCR analysis of 3 TFs on the GSEA gene list which inhibit OC differentiation. Gene expression is shown for WT MPs and OCs and Pu.1 KO OCs (n = 3). g, h Depiction of MP and OC PU.1 and MITF ChIP-Seq peaks near the TF loci analyzed in e and f. Each trace is 30 kb wide and the TSS is indicated. Conventional ChIP validation of PU.1 and MITF binding to the starred sites is shown (bar graphs, n = 3).