Fig. 3.

NDRG2 diminishes CRC cell growth and facilitates cell differentiation. a, b NDRG2 suppresses tumor growth of HT29 cells in nude mice. Mice (five animals per group) were injected s.c. in the right limb with 1 × 10⁷ HT29 cells. Tumors were excised and photographed 21 days after injection a. Tumor size was measured over a 3-week period and tumor volume was calculated by the formula (width² × length × 0.5) b. c–f Cell differentiation status was measured by transmission electron microscopy c, AKP activity analysis d, e, and E-cadherin mRNA expression in the cells labeled on the top of each pane following a 48-h treatment of 2 mM NaBT. g Identification of NDRG2 protein expression in HT29 and HCT116 cells expressing an NDRG2-specific shRNA with or without 2 mM NaBT treatment. h-j Cell differentiation status was measured by AKP activity analysis h, i and E-cadherin mRNA expression j in indicated CRC cells. Data are expressed as means ± SD (n = 3). *P < 0.05