Fig. 4.

NDRG2 elevates p21 and p27 levels. We reduced p21 levels using two-lentivirus expressing p21-specific shRNAs in control or NDRG2-overexpressing HT29 cells. We reduced p27 level using a lentivirus expressing a p27-specific shRNA in control or NDRG2-overexpressing HT29 cells. a, b, e Levels of the indicated proteins were determined by western blotting. c, f AKP activity was determined in each cell population following a 48-h treatment of 2 mM NaBT. d, g E-cadherin mRNA levels were determined by qPCR assay. h, i Mice (four animals per group) were injected s.c. in the right limb with 1 × 10⁷ HT29 cells as indicated. Tumors were excised and photographed 5 weeks after injection, and the tumor weight was recorded h. Tumor size was measured over a 5-week period and tumor volume was calculated by the formula (width² × length × 0.5) i. Data are expressed as means ± SD (n = 3). *P < 0.05