Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Mar 22;9:180. doi: 10.3389/fneur.2018.00180

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018 Almandoz-Gil, Persson, Lindström, Ingelsson, Erlandsson and Bergström.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Representative confocal images of in situ proximity ligation assay (PLA) between alpha-synuclein (αSyn) (mAb1338) and syntaxin-1 (red) in non-tg (A,B) and A30P (C,D) cortical primary neurons. Maximum intensity projections of a confocal z-stack including a whole cell were performed to observe the maximum amount of PLA puncta (A,C). Cross section of the processes allows visualization of the PLA puncta in an orthogonal view (B,D, inlet), scale bar 20 µm. F-actin stained by phalloidin-FITC (green) and DAPI (blue). Quantification of amount of PLA puncta per cell in the soma and processes. One-way ANOVA followed by Bonferroni post hoc test, non-significant (ns), ***P < 0.001. Error bars represent the SEM (E) Negative control PLA with syntaxin-1 antibody only (F). Scale bar 50 µm.