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. 2018 Mar 22;9:180. doi: 10.3389/fneur.2018.00180

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Copyright © 2018 Almandoz-Gil, Persson, Lindström, Ingelsson, Erlandsson and Bergström.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative confocal images of in situ proximity ligation assay (PLA) (red) between h-alpha-synuclein (αSyn) (Syn 204) and VAMP-2 (A,B), h-αSyn (Syn 204) and SNAP-25 (C,D), and h-αSyn (Syn 204) and syntaxin-1 (E,F) in tg A30P neurons and in non-tg neurons (H). Maximum intensity projections of a confocal z-stack including a whole cell were performed to observe the maximum amount of PLA puncta (A,C,E). Cross section of the processes allows visualization of the PLA puncta in an orthogonal view [(B,D,F), inlet], scale bar 20 µm. F-actin stained by phalloidin-FITC (green) and DAPI (blue). Quantification of PLA between h-αSyn and VAMP-2, h-αSyn and SNAP-25, and h-αSyn and syntaxin-1 in A30P neurons (G).